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试剂描述:

产品名称:二硫代苏糖醇

产品别名:DTT

CAS号:27565-41-9

英文别名:二硫代苏糖醇

分子量:154.25

分子式:C₄H₁₀O₂S₂

纯度:>97%

外观:见爱必信官网

保存方法:Store at 4° C

描述:

DTT (1,4-Dithiothreitol) acts as a cell permeable protective agent for Thiol groups. Keeps monothiols completely in the reduced state and reduces disulfides quantitatively. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. Since DTT readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation. Can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. It has been reported that a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol.

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