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产品描述:

产品名称:TMB盐酸盐

CAS号: 207738-08-7

描述:

3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate (TMB) is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that creates a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

中文别名: 3,3’,5,5’-四甲基联苯胺盐酸盐二水合物

外观: 白色或微红色粉末

分子式: C₁₆H₂₀N₂·2HCl·2H₂O

分子量: 349.3

应用: A chromophore substrate in ELISA assays

纯度: 98%

储存/保存方法: Store at 4° C,有效期3年。

产品用途: 科研试剂,测定过氧化氢酶的TMB水溶性底物。

可溶性/溶解性: Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).

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