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产品描述:

产品名称:嘌呤霉素二盐酸盐

CAS号: 58-58-2

描述:

Puromycin is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.

BACKGROUND: Puromycin is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Toxic to both eukaryotic and prokaryotic cells. Believed to act as an acyl-tRNA analogue causing premature chain termination, it has been shown to arrest cells in G2/M phase.

英文别名: Stylomycin hydrochloride;CL 13900 dihydrochloride;CL16536;NSC 3055;P638 dihydrochloride

外观: white to off-white powder

分子式: C₂₂H₂₉N₇O₅•2HCl

分子量: 544.43

应用: An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

纯度: ≥98%

储存/保存方法: Store desiccated at -20° C,3 years.

可溶性/溶解性: Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 7.2 (~10 mg/ml).

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