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- 品牌：爱必信(absin)
- 产地：上海
- 型号：50ul，100ul
- 货号：abs121086
- 价格： ￥1500/支
- 发布日期： 2022-04-08
- 更新日期： 2024-04-25
| 产地 | 上海 |
| 品牌 | 爱必信(absin) |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 货号 | abs121086 |
| 应用范围 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） |
| 抗原来源 | - |
| 保质期 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 规格 | 50ul，100ul |
| 产品用途 | - |
| 抗体名 | NRAS, HRAS, KRAS |
| 是否单克隆 | 否 |
| 克隆性 | 否 |
| 靶点 | - |
| 适应物种 | - |
| 用途范围 | - |
| 形态 | - |
| 宿主 | Rabbit |
| 标记物 | - |
| 亚型 | - |
| 标识物 | - |
| 浓度 | 1mg/ml% |
| 免疫原 | - |
| 是否进口 | 否 |
| 概述 | |
| 别名 |
NRAS抗体, HRAS抗体, KRAS抗体;GTPase NRas, OTTMUSP00000023521, ALPS4, AV095280, HRAS1, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, NRAS1, OTTHUMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog.
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| 宿主 |
Rabbit
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| 特异性 |
NRAS
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| 反应种属 |
Human
|
| 预测反应种属 |
Mouse;Rat;Pig;Cow
|
| 应用 |
ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
|
| 分子量 |
Predicted molecular weight: 21kDa
|
| 免疫原 |
KLH conjugated synthetic peptide derived from human N-Ras:101-189/189
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| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
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| 类型 |
Polyclonal Antibody
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| 同种型 |
IgG
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| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
肿瘤
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| 靶标 | |
| 背景说明 |
N-RAS (neuroblastoma RAS viral oncogene homolog) analogously to other GTP-binding proteins (such as Translation Elongation Factor EFTu or signal transducing G-Proteins) RAS proteins are involved in signal transduction pathways, ras gene family is part of the ras superfamily including the mammalian RAS, RAL, RAC, RHO, RAP, and RAB gene families and the yeast homologs like SEC4 and YPT1 genes; genes encode small monomeric proteins of low molecular mass (20-30 kDa) which share at least 30% homology to RAS proteins.
|
| UniProt |
P01111
|
实验结果图
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: human gastric carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugatedused at 1:200 dilution for 40 minutes at 37°C.
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