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试剂描述:

产品名称:Ac-VDVAD-AFC

产品别名:N-Acetyl-Val-Asp-Val-Ala-Asp-7-amido-4-Trifluoromethylcoumarin;Caspase-2 Substrate (Fluorogenic);

CAS号:210344-94-8

英文别名:Ac-VDVAD-AFC

分子量:770.7

分子式:C₃₃H₄₁F₃N₆O₁₂

纯度:≥95%

外观:见爱必信官网

保存方法:Store at -20℃

描述:Ac-VDVAD-AFC is a fluorogenic substrate whose amino acids VDVAD have been shown to be a preferred cleavage site for caspase-2.^{1 ,2} Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. Increases in Ac-VDVAD-AFC cleavage have been reported to correlate with nitric oxide-induced activation of caspase-2 and subsequent apoptosis in human neuroblastoma lines.¹

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