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产品展厅
UltraNuclease Protein
  • 品牌:Absin
  • 产地:上海
  • 货号:abs60540
  • 价格: ¥420/瓶
  • 发布日期: 2023-09-14
  • 更新日期: 2024-02-21
产品详请
产地 上海
品牌 Absin
货号 abs60540
用途 -
产品规格 25KU;50KU
纯度 >95% by SDS-PAGE%
是否进口

产品描述:

Multi Nuclease all-around nuclease, also called broad-spectrum nucleic acid enzyme, is a kind of comes from Serratia Marcescens restriction endonuclease. It is capable of degradation of all forms of DNA and RNA (double-stranded, single-stranded, linear, circular or superhelical forms) under a very wide range of conditions (6Murea, 0.1M GuanidineHCl, 0.4%TritonX100, 0.1%SDS, 1mM EDTA, 1mM PMSF). The formation of 3-5 oligonucleotide residues containing 5 '-phosphate terminus is widely used to remove nucleic acids from biological products. The expression and purification of this product in Escherichia coli(E.coli) through genetic engineering can not only reduce the viscosity of cell supernatant and cell lysate in scientific research, but also improve the efficiency of protein purification and functional research. It can also be used in virus purification, vaccine production, protein and polysaccharide pharmaceutical industry as a host residual nucleic acid removal reagent, reducing the host residual nucleic acid to the peak (pg) level to improve the efficacy and safety of biological products. And can effectively prevent human peripheral blood monocyte (PBMC) clumping in cell therapy and vaccine research.


Component:

Components Amount
UltraNuclease* 25000U/ml
Buffer Formulation 10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol
* One unit of Nuclease is defined as the amount of enzyme that causes a ?A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min.

来源:E.coli

序列:

Asp22-Asn266
DTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN

活性:25000U/mL

使用方法:

1.Sample preparation:
Adherent cells: Remove the medium, clean the cells with PBS, and remove the supernatant.
Suspension cells: Cells were collected by centrifugation, cleaned with PBS, centrifuged at 6,000rpm for 10min, and precipitates were collected.
Escherichia coli: The bacteria were collected by centrifugation, cleaned once with PBS, centrifuged at 8,000rpm for 5min, and precipitates were collected.

2.Sample treatment:
The collected cell precipitates are cleaved according to the ratio of mass (g) to volume (mL) to 1: (10-20). Cells can also be cleaved mechanically or chemically on ice or at room temperature (1g cells are about 109).

3.Enzyme addition:
the proportion of 1g cell precipitation digested by 250Units is required. You can also choose the addition plan according to the recommended dosage in the table above, increase the amount of enzyme within a certain range, and reduce the digestion time accordingly.

4.Supernatant acquisition:
The supernatant of cell lysis solution was obtained by centrifugation at 12,000rpm for 30min, and then subsequent related experiments were conducted.

Conditional parameter Optimum condition Applicable condition
Mg2+ 1-2mM 1-10mM
pH 8.0 6-10
Temperature 37℃ 0-42℃
DTT 0-100mM >0mM
β-Me 0-100mM >0mM
Monovalent cation 0-200mM 0-150mM
Phosphate anion 0-10mM 0-100mM

储存/保存方法

Within 1 week, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.

技术指标:

Accession: P13717;
种属: Serratia marcescens;
标签: His Tag

形态:

10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol

实验结果图:

M marker 1:0.2g/ml Escherichia coli lysate.2:0.2g/ml Escherichia coli lysate+1μl well-known UltraNuclease.3:0.2g/ml Escherichia coli lysate+1μl UltraNuclease.

M marker 1:10μg Salmon sperm DNA.2:37μg Salmon sperm DNA+0.5U UltraNuclease.3:37μg Salmon sperm DNA+0.75U UltraNuclease.4:37μg Salmon sperm DNA+1U UltraNuclease.

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